IP Responsible: Prof Carlos Simón
Phd Student: Tamara Garrido
Lab Technician: Alicia Quiñonero
Research Description
Currently, to evaluate the state of the endometrium of a woman there are a few techniques with little objective like the histological appraisal, based on observations described by Noyes, for over 50 years, or macroscopic techniques as ultrasound and hysteroscopic little decisive and likewise subjective. This project intends to determine with precision the protean profile of the human endometrial responsiveness through the analysis of samples of endometrium in different situations.. A first study will compare the protean profile of the non receptive endometrium against the endometrium in receptive state, which corresponds to the so-called "window of implantation", during the which, the endometrium is going to be receptive to the embryo implantation.. It is a matter of knowing the proteins that are present in greater or smaller quantity in a situation with regard to the other. For it, biopsies of endometrium and samples of endometrial fluid samples of 5 women in their natural cycle will be taken.. For the analysis of the endometrium in a non receptive state, it will be used samples of LH 2, that corresponds to the second day after the LH peak, and for the receptive samples of LH 7 of the same donor, which corresponds to the seventh day after the said peak. A subsequent objective would be the design of a predictor that permit to evaluate in an objective form, the receptive state of an endometrium (PRE, Predictor of endometrial responsiveness) from a sample of uterine secretion.. Further studies will be the analysis of protein profile along the natural cycle, as well as in different situations, like stimulated cycle, with treatment with oral contraceptive, with therapy of hormonal replacement, diseases such as endometriosis etc.. Our objectives in this project are:
Determine with precision the protean profile of the human endometrial responsiveness through the analysis of samples of endometrium in different situations.
Specific objectives
- Determine the differential protein profile of the endometrium in pre-receptive state against receptive endometrium (implantation window).
- Determine the protein profile of the endometrium during the natural cycle as well as in stimulated cycle, with treatment with oral contraceptive, with therapy of hormonal replacement,diseases such as endometriosis etc.
- Design a predictor to assess, in an objective form, the receptive state of an endometrium (PRE, predictor of endometrial receptivity) from a sample of uterine secretion.
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