Francisco DomínguezPhd Student:
Tamara GarridoLab. Tecchnician:
Alicia Quiñonero y Maria Herrero
Nowadays we don’t have objective tools to evaluate the endometrial status other tan subjective histological evaluation, based on observations described by Noyes and colleagues in the 50s, or macroscopic techniques such as ecography or hysteroscopy. With this project we intend to precisely determine the human endometrial protein profile using proteomics techniques and different endometrial samples. Our final objective should be to design a predictor trough an endometrial fluid sample, less invasive than endometrial biopsy. Other future studies would include the endometrial proteomic profile of a hormonal replacement cycle, oral contraceptives or diseases such as the endometriosis.
Proteomic network of deregulated proteins between receptive and non-receptive endometria diagnosed by endometrial receptivity array. All proteins are represented by their gene symbol name. Different colour lines connecting proteins represent the relationships between them (physical interaction, co-expression, co-localization, shared and predicted protein domains). Spheres represent proteins obtained in our proteomic study while squares represent link nodes between them. Two statistically significant pathways were identified in the network: proteins integrated in the ‘carbohydrate biosynthetic process’ are represented in orange while proteins related to ‘nuclear mRNA splicing via spliceosome’ are represented in orange
- To determine the proteomic protein profile of pre-receptive and receptive endometria (window of implantation).
- To determine the responsible proteins for a correct acquisition of the endometrial receptivity.
- To determine the protein profile throughout the menstrual cycle, and pathological conditions such as implantation failure, endometriosis or pre-eclampsia.
- To design a predictor to objectively evaluate the receptive status of the endometrium.
Domínguez F, Garrido-Gómez T, López JA, Camafeita E, Quiñonero A, Pellicer A, Simón C.
Proteomic analysis of the human receptive versus non-receptive endometrium using differential in-gel electrophoresis and MALDI-MS unveils stathmin 1 and annexin A2 as differentially regulated. Hum Reprod. 24(10):2607-17. (2009)
Garrido-Gómez T, Dominguez F, Simón C.
Proteomics of embryonic implantation.Handb Exp Pharmacol. 2010;(198):67-78.
Garrido-Gomez T, Dominguez F, Lopez JA, Camafeita E, Quiñonero A, Martinez-Conejero JA, Pellicer A, Conesa A, Simón C.
Modeling human endometrial decidualization from the interaction between proteome and secretome. J Clin Endocrinol Metab. 2011 Mar;96(3):706-16.
T Garrido-Gómez, F Dominguez, A Quiñonero, C Estella, F Vilella, A Pellicer and C Simon.
Annexin A2 is critical for embryo adhesiveness to the human endometrium by RhoA activation through F-actin regulationFASEB J. 2012 Sep;26(9):3715-27
Domínguez F, Simón C, Quiñonero A, Ramírez MÁ, González-Muñoz E, Burghardt H, Cervero A, Martínez S, Pellicer A, Palacín M, Sánchez-Madrid F, Yáñez-Mó M.
Human endometrial CD98 is essential for blastocyst adhesion. PLoS One. 2010 Oct 15;5(10):e13380. doi: 10.1371/journal.pone.0013380.
Thouas GA, Dominguez F, Green MP, Vilella F, Simon C, Gardner DK.
Soluble ligands and their receptors in human embryo development and implantation. Endocr Rev. 2015; 36(1):92-130. 2014.
Garrido-Gómez T, Quiñonero A, Antúnez O, Díaz-Gimeno P, Bellver J, Simón C, Domínguez F.
Deciphering the proteomic signature of human endometrial receptivity.Hum Reprod ;29(9):1957-67. 2014.
Deciphering the proteomic signature of human endometrial receptivity. Garrido-Gómez T, Quiñonero A, Antúnez O, Díaz-Gimeno P, Bellver J, Simón C, Domínguez F.
Hum Reprod. 2014 Sep;29(9):1957-67. doi: 10.1093/humrep/deu171. Epub 2014 Aug 8.
Dominguez F, Moreno-Moya JM, Lozoya T, Romero A, Martínez S, Monterde M, Gurrea M, Ferri B, Núñez MJ, Simón C, Pellicer A
Embryonic miRNA profiles of normal and ectopic pregnancies. PLoS One. 2014 ;9(7):e102185. 2014.
Dominguez F, Meseguer M, Aparicio-Ruiz B, Piqueras P, Quiñonero A, Simón C.
New strategy for diagnosing embryo implantation potential by combining proteomics and time-lapse technologies. Fertil Steril. 2015 Oct;104(4):908-14.
Número de proyecto/contrato: PI12/00450
Título del proyecto/contrato: Descripción del perfil proteómico endometrial del Fallo de Implantación recurrente. Creación del interactoma del fallo de implantación
Empresa/Administración financiadora: ISCA - Instituto de Salud Carlos III
Duración: desde: 2013 hasta: 2015
Investigador/a Principal: Francisco Dominguez