Proteimics Endometrial
Proteimics Endometrial

(Francisco Dominguez Ph.D)

Currently, to evaluate the state of the endometrium of a woman there are a few techniques with little objective like the histological appraisal...

Testicular stem cells
Testicular stem cells

(Carlos Simón MD, PhD)

Sperm generated in the adult male gonads from a small germinal stem cell population..

Endometrial and myometrial stem cells
Endometrial and myometrial stem cells

 (Irene Cervelló Alcaraz, PhD)

The exceptional capacity of human endometrial tissue regeneration, that is renewed monthly and that can do it during all the life...

Endometrial receptivity
Endometrial receptivity

(Felipe Vilella, PhD)

The endometrium is the surface tissue that cover the walls of the uterus. In humans, this tissue suffers physiological and morphological modifications along the menstrual cycle...

Endometriosis
Endometriosis

(Francisco Domínguez PhD)

Endometriosis is one of the most frequent bening gynecological diseases in women, which may cause infertility.

Ovarian hiperstimulation
Ovarian hiperstimulation

(Antonio Pellicer Martínez MD, PhD)

During assisted reproduction cycles and in order to obtain a higher number of oocytes women are administered Gonadotrophins....

Embryonic viability
Embryonic viability

 (Francisco Dominguez, PhD)

Nowadays the unique method used to select the most viable embryo with the greatest potential in IVF implantation ...

 

Reprograming
Reprograming

 (José V. Medrano, PhD)

Infertility affects up to 15% of couples in reproductive age in Europe. Among the different diseases that can lead to infertility

Vitamine D treatment on the uterine fibroids growth
Vitamine D treatment on the uterine fibroids growth

 Uterine fibroids are the most common benign tumor in women of reproductive age, affecting 25% of them...

Endometrial Database
Endometrial Database

Universidad de Valencia

EDB is a service of Fundacion IVI, sponsored by University of Valencia (SPAIN)


Testicular Stem Cells

Investigador
P.I. Responsible: Carlos Simón, MD, PhD
PhD student: Marcia Riboldi
Lab. Technician: Sebastian Martinez

Research Description

Obtaining human cell lines from testicular biopsies for preservation of the fertility.

Sperm generated in the adult male gonads from a small germinal stem cell population. This gametes formation process, called spermatogenesis is initiated in the puberty, although its origin is already established during fetal development, when present immature germinal cells in the space extraembryonic proliferate and emigrate to the interior of the gonads. Here, the germinal cells are lodged in the basal membrane of the seminiferous tubules and they differentiate toward spermatogonial, which maintain their capacity of proliferation and of gamete formation during all the life of the individual. Recent studies show the presence of pluripotent cells in the adult human testicle. The importance of these cells resides in their capacity to perpetuate indefinitely and to maintain the capacity of differentiation toward spermatozoa, conserving therefore their characteristics of germinal origin. These cells can be isolated in the laboratory from testicular biopsies making use of antibodies and selective matrices for further cultivation, expanded and maintained in vitro for extended periods of up to 6 months. The present project intends to generate pluripotent human germ line from testicular biopsies carried out in a routine way in patients with obstructive azoospermia, and to test its capacity for the processing of the Male Infertility. The medium-term objective is to develop a methodology that can be offered children affected by cancer submitted to chemotherapy or radiation therapy, which causes an irreversible stop in the production of spermatozoa and therefore infertility. Testicular pluripotent cell lines obtained from the patient before the treatment could be used in autologous transplants after the therapy to revive the spermatogenesis, surpassing thus the infertility caused by the anticancer treatment.

Posted Items
  • Riboldi, M. Simon, C.

    Extraembryonic tissues as a source of stem cells.
    Gynecological Endocrinology (2009) 25(6): 351–355.

  • Medrano JV , Marqués-Marí AI , Aguilar CE , Riboldi M , Garrido N, Martínez-Romero A, O´Connor E, Gil-Salom M and Simón C.

    Comparative analysis of the germ cell markers c-KIT, SSEA-1 and VASA in testicular biopsies from secretory and obstructive azoospermia.
    Molecular Human Reproduction (In press)

Book Chapters
  • Marcia Riboldi, Ana Isabel Marqués Marí, Carlos Simón

    Stem cells and fertility preservation in male.
    Fertility Preservation by Humana Press (2010).