Proteimics Endometrial
Proteimics Endometrial

(Francisco Dominguez Ph.D)

Currently, to evaluate the state of the endometrium of a woman there are a few techniques with little objective like the histological appraisal...

Testicular stem cells
Testicular stem cells

(Carlos Simón MD, PhD)

Sperm generated in the adult male gonads from a small germinal stem cell population..

Endometrial and myometrial stem cells
Endometrial and myometrial stem cells

 (Irene Cervelló Alcaraz, PhD)

The exceptional capacity of human endometrial tissue regeneration, that is renewed monthly and that can do it during all the life...

Endometrial receptivity
Endometrial receptivity

(Felipe Vilella, PhD)

The endometrium is the surface tissue that cover the walls of the uterus. In humans, this tissue suffers physiological and morphological modifications along the menstrual cycle...

Endometriosis
Endometriosis

(Antonio Pellicer Martínez MD, PhD)

The endometriosis is an estrogen-dependent gynecological disorder of idiopathic origin and functional disability associated with infertility in women in reproductive age ...

Ovarian hiperstimulation
Ovarian hiperstimulation

(Antonio Pellicer Martínez MD, PhD)

During assisted reproduction cycles and in order to obtain a higher number of oocytes women are administered Gonadotrophins....

Embryonic viability
Embryonic viability

 (Francisco Dominguez, PhD)

Nowadays the unique method used to select the most viable embryo with the greatest potential in IVF implantation ...

 

Reprograming
Reprograming

 (José V. Medrano, PhD)

Infertility affects up to 15% of couples in reproductive age in Europe. Among the different diseases that can lead to infertility

Endometrial Database
Endometrial Database

Universidad de Valencia

EDB is a service of Fundacion IVI, sponsored by University of Valencia (SPAIN)


Endometrial Stem Cells

P.I. Responsible: Irene Cervelló Alcaraz, PhD | Carlos Simón MD, PhD
PhD Student: Aymara Mas Perucho | Claudia Gil Sanchis
Lab Technician: Amparo Faus




 

 

Research Description

The exceptional capacity of human endometrial tissue regeneration, that is renewed monthly and that can do it during all the life with an appropriate hormonal treatment, makes it a major tissue for the investigation of its population of somatic stem cells that up to now have not been described. The present project intends to identify, to locate, to characterize and to isolate this cell population of great interest for the understanding of the ometrial physiology and pathology. The obtainment of somatic stem cells offers a promising and excellent therapeutic power. The benefits that this project can generate in Regenerative Medicine, infertility-related diseases and, potentially in endometrial cancer are enormous for being one of the most innovative biotechnology tools that exist today. Recently, it has been suggested in different publications the somatic mother cells presence or progenitor cells in human endometrium and its implication in the reconstruction of this tissue, acting together with the molecular and cell factors cited previously ( Figure 1 suggests a possible location of endometrial stem cells in the human endometrium CME ). Prior results obtained from an animal model with the BrdU (Publication in Human Reproduction) permit us to conclude the somatic mother cells existence in the mouse endometrium (Figure 2). Currently we are employing the elaborate protocols by our investigator group for the detection On the flow cytometer of the Side Population (SP), undifferentiated population of cells already detected in other tissues as bone marrow by their ability to expel a vital dye (Hoechst 33342), both in endometrium of mouse and humans. This will permit to identify and to locate a population of cells undifferentiated cells present in these tissues. The results obtained up to now in the endometrial permit to identify a population of cells SP in human and in the stromal compartment as in the epitelial (Figure 3). From endometrial samples we obtain the two major cell types of human endometrium: the epitelio and the estroma. Once obtained the Side Population, cell suspensions isolated from epithelium and stroma were analyzed through molecular analysis and inmucitoquímicos being shown that the cells of the SP versus the NSP (odd-SP) of human endometrium express marker of differentiation and SP typical cell suggesting a possible enrichment of somatic stem cells. The activity telomerasa of the SP contained a characteristic intermediate boss among cells indiferenciadas (cells embryonic mother) and differentiated cells (endometrial cells), indicating again that this population of cells SP would be able to contain a madre somatic cell sub-population.. Beyond the cells from their differentiated SP in vitro by means of protocols already established to tissues from mesenchymal lineages as osteocytes and adipocytes, being shown thus its possible mesenchymal origin. Our team of investigation has also managed to reconstruct human endometrial glands through the subcutaneous injection of these cells SP candidates in immunodeficient mice (NOD-SCID) (Publication in Plos One). Part of this work developed in the human model has been rewarded in the Company of Gynecological Investigation (SGI) in 2009- The Wyeth President' s Presenter Award was established in 1996 and recognizes the 25 best abstracts or summaries of works (in 2009 from among 1056) presented in the SGI by researchers in the formation phase.

 

Scientific Publications.

 

INTERNATIONALS:

 

  • Cervelló I, Martínez-Conejero JA, Horcajadas JA, Pellicer A, Simón C.

“Identification, characterization and co-localization of label-retaining cell population in mouse

endometrium with typical undifferentiated markers”.

Human Reproduction 2007; 22(1). 

 

  • Cervelló I, Simón C.

“Somatic Stem cells in the endometrium”.

Reproductive Sciences 2009;16(2). 

 

  • Cervelló I,Gil-Sanchis C, Mas A, Simón C.

“Current understanding of Endometrial Stem cells”.

Expert Review of Obstetrics & Gynecology 2009;Vol. 4(3). 

 

  • Cervelló I, Gil-Sanchis C, Mas A, Delgado-Rosas F, Martínez-Conejero JA, Galán A,

Martínez-Romero A, Martínez S, Navarro I, Ferro J, Horcajadas JA, Esteban FJ, O'Connor JE, Pellicer A, Simón C. 

“Human endometrial Side Population exhibit genotypic, phenotypic and functional features of somatic stem cells”.

PLoS One 2010 24;5(6). 

 

  • Cervelló I, Mas A, Gil-Sanchis C, Peris L, Faus A, Saunders PT, Critchley HO, Simón C.

Reconstruction of endometrium from human endometrial side population cell lines.

PLoS One. 2011;6(6):e21221 

 

  • Cervelló I, Mirantes C, Santamaria X, Dolcet X, Matias-Guiu X, Simón C.

“Stem cells in human endometrium and endometrial carcinoma”.

Int J Gynecol Pathol. 2011 Jul;30(4):317-27. 

 

  • Cervelló I, Gil-Sanchis C, Mas A, Faus A, Sanz J, Moscardó F, Higueras G, Sanz MA, Pellicer A and Simón C.

“Bone marrow-derived cells from male donors do not contribute to the endometrial side

population of the recipient”.

PLoS One. 2012;7(1):e30260. 

 

  • Mas A, Cervelló I, Gil-Sanchis C, Faus A, Ferro J, Pellicer A, Simón C.

“Identification and characterization of the human leiomyoma side population as putative tumor-initiating cells”. 

Fertil Steril. 2012;98(3):741-751. 

 

  • Cervelló I, Mas A, Gil-Sanchis C, Simón C. 

“Somatic stem cells in the human endometrium”. 

Semin Reprod Med. 2013 Jan;31(1):69-76.

 

  • Gil-Sanchis C, Cervelló I, Mas A, Faus A, Pellicer A, Simón C.

“Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) as a putative human endometrial stem cell marker”. 2013. Mol Hum Reprod. 

 

NATIONALS:

 

  • Cervelló I, Pellicer A. y Simón C.

Células madre adultas en el útero.

Cuadernos de Medicina Reproductiva. Nuevos avances en Medicina Reproductiva.

Volumen 12 Número 2, 2006. Pages 73-78. ISSN 1135-0970

 

  • Romeu M, Cervelló I, Sánchez M., Pellicer A. y Simón C.

Células madre adultas en ovario. Una hipótesis atractiva.

Cuadernos de Medicina Reproductiva. Nuevos avances en Medicina Reproductiva.

Volumen 12 Número 2, 2006. Pages 53-59. ISSN 1135-0970

 

Contributions to Books.

INTERNATIONALS:

 

  • Gargett C, Cervelló I, Hubbard S and Simón C.

Stem Cells in Human reproduction. Basic Science and Therapeutic potential. Second Edition.

Chapter: Adult Stem Cells in the Human Endometrium.

Pages 160-176

Informa Healthcare.2009. ISBN 978 0 4154 7171 2

 

  • Gargett C, Cervelló I, Nguye P.T, and Simón C.

Stem Cells in Reproductive Medicine: Basic Science and Therapeutic Potential, 3rd Edition.

Chapter 21: Adult Stem Cells in the Human Endometrium

In Press.

Cambridge University Press.

 

  • Cervelló I y Simón C.

Advances in Fertility studies and Reproductive Medicine. IFFS 2007.

Chapter: Adult Stem Cells in the Uterus.

Pages 161-166.

Besins Healthcare. 2007. ISBN 978 07021 767222

 

NATIONALS:

 

  • Cervelló I y Simón C.

El Endometrio Humano. Desde la Investigación a la Clínica.

Chapter: Células madre adultas en el endometrio.

Pages 183-189.

Editorial Medica Panamericana. 2009. ISBN 978 84 9835 089 0